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96
Proteintech antibodies against fdx1
Effect of <t>FDX1</t> overexpression on the proliferation and apoptosis levels of COAD cells. (A) Morphological changes in HCT116 and LoVo cells after overexpressing FDX1. Scale bar is set at 100 μm. (B, C) CCK-8 assay evaluated proliferation rates of HCT116 and LoVo cells after overexpressing FDX1. (D, E) Colony formation assay of HCT116 and LoVo cells after overexpressing FDX1. (F) EdU proliferation assay assessed proliferation levels of HCT116 and LoVo cells after overexpressing FDX1. (G) Flow cytometry analysis of apoptosis levels in HCT116 and LoVo cells after overexpressing FDX1. *** p < 0.001.
Antibodies Against Fdx1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Effect of FDX1 overexpression on the proliferation and apoptosis levels of COAD cells. (A) Morphological changes in HCT116 and LoVo cells after overexpressing FDX1. Scale bar is set at 100 μm. (B, C) CCK-8 assay evaluated proliferation rates of HCT116 and LoVo cells after overexpressing FDX1. (D, E) Colony formation assay of HCT116 and LoVo cells after overexpressing FDX1. (F) EdU proliferation assay assessed proliferation levels of HCT116 and LoVo cells after overexpressing FDX1. (G) Flow cytometry analysis of apoptosis levels in HCT116 and LoVo cells after overexpressing FDX1. *** p < 0.001.

Journal: Frontiers in Cell and Developmental Biology

Article Title: Assessing the prognostic and therapeutic value of cuproptosis-related genes in colon adenocarcinoma patients

doi: 10.3389/fcell.2025.1550982

Figure Lengend Snippet: Effect of FDX1 overexpression on the proliferation and apoptosis levels of COAD cells. (A) Morphological changes in HCT116 and LoVo cells after overexpressing FDX1. Scale bar is set at 100 μm. (B, C) CCK-8 assay evaluated proliferation rates of HCT116 and LoVo cells after overexpressing FDX1. (D, E) Colony formation assay of HCT116 and LoVo cells after overexpressing FDX1. (F) EdU proliferation assay assessed proliferation levels of HCT116 and LoVo cells after overexpressing FDX1. (G) Flow cytometry analysis of apoptosis levels in HCT116 and LoVo cells after overexpressing FDX1. *** p < 0.001.

Article Snippet: Antibody against Lipoic Acid (ab58724) was sourced from Abcam, while antibodies against FDX1 (12592-1-AP) and β-actin (66009-1-Ig) were purchased from Proteintech.

Techniques: Over Expression, CCK-8 Assay, Colony Assay, Proliferation Assay, Flow Cytometry

Effect of elesclomol treatment combined with FDX1 overexpression on the proliferation and apoptosis levels of COAD cells. (A) Morphological changes in HCT116 and LoVo cells after overexpression of FDX1. Scale bar is set at 100 μm. (B, C) CCK-8 assay evaluated proliferation rates of HCT116 and LoVo cells in Control, ES, ES + pcDNA-NC, and ES + pcDNA-FDX1 groups. (D, E) Colony formation assay of HCT116 and LoVo cells in Control, ES, ES + pcDNA-NC, and ES + pcDNA-FDX1 groups. (F, G) Flow cytometry analysis of apoptosis levels in HCT116 and LoVo cells from Control, ES, ES + pcDNA-NC, and ES + pcDNA-FDX1 groups. *** p < 0.001.

Journal: Frontiers in Cell and Developmental Biology

Article Title: Assessing the prognostic and therapeutic value of cuproptosis-related genes in colon adenocarcinoma patients

doi: 10.3389/fcell.2025.1550982

Figure Lengend Snippet: Effect of elesclomol treatment combined with FDX1 overexpression on the proliferation and apoptosis levels of COAD cells. (A) Morphological changes in HCT116 and LoVo cells after overexpression of FDX1. Scale bar is set at 100 μm. (B, C) CCK-8 assay evaluated proliferation rates of HCT116 and LoVo cells in Control, ES, ES + pcDNA-NC, and ES + pcDNA-FDX1 groups. (D, E) Colony formation assay of HCT116 and LoVo cells in Control, ES, ES + pcDNA-NC, and ES + pcDNA-FDX1 groups. (F, G) Flow cytometry analysis of apoptosis levels in HCT116 and LoVo cells from Control, ES, ES + pcDNA-NC, and ES + pcDNA-FDX1 groups. *** p < 0.001.

Article Snippet: Antibody against Lipoic Acid (ab58724) was sourced from Abcam, while antibodies against FDX1 (12592-1-AP) and β-actin (66009-1-Ig) were purchased from Proteintech.

Techniques: Over Expression, CCK-8 Assay, Control, Colony Assay, Flow Cytometry

(A, B) EdU proliferation assay with HCT116 and LoVo cells in Control, ES, ES + pcDNA-NC, and ES + pcDNA-FDX1 groups. Scale bar is set at 50 μm * p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: Frontiers in Cell and Developmental Biology

Article Title: Assessing the prognostic and therapeutic value of cuproptosis-related genes in colon adenocarcinoma patients

doi: 10.3389/fcell.2025.1550982

Figure Lengend Snippet: (A, B) EdU proliferation assay with HCT116 and LoVo cells in Control, ES, ES + pcDNA-NC, and ES + pcDNA-FDX1 groups. Scale bar is set at 50 μm * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Antibody against Lipoic Acid (ab58724) was sourced from Abcam, while antibodies against FDX1 (12592-1-AP) and β-actin (66009-1-Ig) were purchased from Proteintech.

Techniques: Proliferation Assay, Control

Effect of elesclomol combined with FDX1 overexpression on cuproptosis levels in COAD cells. (A–D) Western blot analysis of lipoylated DLAT (lip-DLAT) and FDX1 expression of HCT116 and LoVo cells in the Control, ES, ES + pcDNA-NC, and ES + pcDNA-FDX1 groups. (E) The fluorescence intensity of HCT116 and LoVo cells in Control, ES, ES + pcDNA-NC, and ES + pcDNA-FDX1 groups was observed using confocal microscopy after staining with the mitochondrial dye TMRE. Scale bar is set at 40 μm. (F, G) Flow cytometry analysis of mitochondrial membrane potential levels in HCT116 and LoVo cells after TMRE staining in Control, ES, ES + pcDNA-NC, and ES + pcDNA-FDX1 groups. *** p < 0.001.

Journal: Frontiers in Cell and Developmental Biology

Article Title: Assessing the prognostic and therapeutic value of cuproptosis-related genes in colon adenocarcinoma patients

doi: 10.3389/fcell.2025.1550982

Figure Lengend Snippet: Effect of elesclomol combined with FDX1 overexpression on cuproptosis levels in COAD cells. (A–D) Western blot analysis of lipoylated DLAT (lip-DLAT) and FDX1 expression of HCT116 and LoVo cells in the Control, ES, ES + pcDNA-NC, and ES + pcDNA-FDX1 groups. (E) The fluorescence intensity of HCT116 and LoVo cells in Control, ES, ES + pcDNA-NC, and ES + pcDNA-FDX1 groups was observed using confocal microscopy after staining with the mitochondrial dye TMRE. Scale bar is set at 40 μm. (F, G) Flow cytometry analysis of mitochondrial membrane potential levels in HCT116 and LoVo cells after TMRE staining in Control, ES, ES + pcDNA-NC, and ES + pcDNA-FDX1 groups. *** p < 0.001.

Article Snippet: Antibody against Lipoic Acid (ab58724) was sourced from Abcam, while antibodies against FDX1 (12592-1-AP) and β-actin (66009-1-Ig) were purchased from Proteintech.

Techniques: Over Expression, Western Blot, Expressing, Control, Fluorescence, Confocal Microscopy, Staining, Flow Cytometry, Membrane